Animal Bones Research Articles

The feasibility of K XRF bone lead measurements in mice assessed using 3D-printed phantoms

This article describes the development of a system for in vivo measurements of lead body burden in mice using 109Cd K x-ray fluorescence (XRF). This K XRF system could facilitate early-stage studies on interventions that ameliorate or reverse organ tissue damage from lead poisoning by reducing animal numbers through a cross-sectional study approach. A novel mouse phantom was developed based on a mouse atlas and 3D-printed using PLA plastic with plaster of Paris ’bone’ inserts. PLA plastic was found to be a good surrogate for soft tissue in XRF measurements and the phantoms were found to be good models of mice. As expected, lead detection limits varied with mouse size, mouse orientation, and mouse position with respect to the source and detector. The work suggests that detection limits of 10 to 20 μg Pb per g bone mineral may be possible for a 2 to 3 hour XRF measurement in a single animal, an adequate limit for some pre-clinical studies. The 109Cd K XRF mouse measurement system was also modeled using the Monte Carlo code MCNP. The combination of experiment and modeling found that contrary to expectation, accurate measurements of lead levels in mice required calibration using mouse-specific calibration standards due to the coherent scatter peak normalization failing when small animals are measured. MCNP modeling determined that this was because the coherent scatter signal from soft tissue, which until now has been assumed negligible, becomes significant when compared to the coherent scatter signal in bone in small animals. This may have implications for some human measurements. This work suggests that 109Cd K x-ray fluorescence measurements of lead body burden are precise enough to make the system feasible for small animals if appropriately calibrated. Further work to validate the technology’s measurement accuracy and performance in vivo will be required.

Two-stage repeat sintering: A modified method for fabricating hydroxyapatite scaffolds with enhanced mechano-therapeutic properties

A modified two-stage repeat sintering (TSRS) technique has been used to produce hydroxyapatite (HAp) from non-separated animal bones, and the energy consumption per cycle of the proposed TSRS was calculated. The TSRS technique was compared to the conventional one-step sintering method. The energy consumption per cycle of the proposed TSRS was calculated considering economies of scale and typical energy consumption billing in Nigeria. The synthesized powders from both techniques underwent comprehensive characterization through X-ray diffraction (XRD), Fourier transform infrared (FT-IR), and scanning electron microscopy (SEM). The XRD analysis revealed the main reflections of HAp in the (112), (300), and (202) planes. The FT-IR spectra exhibited characteristic bands representative of HAp at all measurement points. Notably, the TSRS-derived sample during the second sintering step showed carbonate bands, suggesting relevance in the bone structure makeup. SEM images unveiled varying crystal morphologies, ranging from irregular shapes in the raw bone (RB) sample to rounded shapes with nucleation in aggregates and agglomerates at micro and macro levels in the TSRS-derived samples. The samples obtained from the TSRS technique demonstrated a high Ca/P ratio that is potentially beneficial for osteoblast adhesion in vivo. The cost analysis of electricity consumption per TSRS cycle, factoring in economies of scale and typical energy consumption billing in Nigeria, amounted to approximately $8 with consideration of the exchange rates at the time of the study. The microhardness measured using the TSRS technique was 0.5 GPa with a 1 MPa compaction pressure, while the samples prepared using the conventional sintering method were too fragile to handle and measure. The obtained microhardness using the modified technique surpassed values reported in various studies. In conclusion, the samples produced using the TSRS technique exhibited promising results regarding energy efficiency, material characteristics, and microhardness compared to the samples produced through conventional sintering. The distinct crystal morphologies observed suggest potential advantages for biomedical applications, particularly in enhancing osteoblast adhesion.

Impacts of working bone and antler on a bronze knife: Experimental and use-wear studies

To answer the question if metal knives found in late Bronze Age deposits in Karmin in W Poland (ca. 900–800 BCE) could have been used as multi-tools in processing hard materials like red deer antler and animal bone, we applied an experimental method followed by the use-wear analysis of a replica of late Bronze Age bronze knife. This is the first experiment that focuses on the tool instead of the processed material. It included five movements engaging various sections of a knife blade and tip and producing diversified traces depending on the type of technique and raw material worked. We showed that an adequately cast knife, hardened then by cold working, could have been applied in all stages of manufacturing antler and bone objects, from initial material division (cross-cutting) over shaping (surface cutting, whittling) to finishing (scraping, drilling). Although the tool required frequent resharpening, it efficiently performed various movements. The traces on the replica, such as U-shaped notches, chips, blunting, bows, scratches, and serrated and wavy edges, correspond well with those observed on the knives from Karmin.

Effect of bone age and anatomy on the variability of the bovine bone by-product by Terahertz time-domain spectroscopy and energy-dispersive X-ray microanalysis

Bone is among the main by-products of the meat and dairy livestock. Currently, in many countries, it is valorised in pet food, disposed through incineration or exported. However, thanks to its biological and mechanical properties, it could be used in several bio-based products such as coatings, packaging, phosphorous, calcium and magnesium supply, flame retardants, etc. Successful valorisations routes of discarded bones need a comprehensive characterization of the bone tissue variability with major biological factors such as age of the animal and the anatomy of the bone itself. In this study, this variability was assessed through Terahertz time-domain spectroscopy and energy dispersive X-ray microanalysis. Bones optical properties (refractive index and absorption coefficient) and main minerals (calcium and phosphorous), were determined for adult cows’ femurs and tibias, from the same breed and breeding system and in the age range 93–120 months. Clustering analysis was then carried out, by unsupervised statistical tools (Principal Component Analysis and Agglomerative Hierarchical Clustering). Results showed that the bones considered can be grouped in two classes, represented by each anatomy; besides, the anatomy was most significant than age. The refractive index increased with age and was higher for femur; the cluster of tibias was more homogeneous than femurs. The THz radiation showed to be a useful and non-destructive tool to assess bovine bones variability and also their optical properties. This radiation could be applied to the predict animal bone components (collagen and minerals) extractability so as to valorise each cluster according to its potential.

Early history of the study of bone growth (1722-1875).

Bone growth is a fascinating process, primarily due to its complexity. Equally engaging is the history of its study, which, however, remains unknown to most anatomists and surgeons. A literature search was performed in original publications and historical sources. The early history of bone growth study may be divided into two periods. Firstly, the experimental one, between 1722 and 1847, which consisted in the study of bone growth by the drilling of benchmark holes into the diaphysis, and examination of growing bones in madder-fed animals. In the course of one century, four French scientists (Henri-Louis Duhamel du Monceau, Marie-Jean-Pierre Flourens, Gaspard Auguste Brulléand Frédéric Léopold Hugueny) and one British researcher (John Hunter) proved experimentally that the longitudinal growth of long bones occurred only at its epiphyseal ends and their final shape resulted from apposition and resorption processes taking place simultaneously both on the periosteal and intramedullary surfaces of the bone. In the second, the microscopic period (1836-1875), the physeal growth cartilage was discovered and described in detail, including its importance for the longitudinal growth of long bones. The first description of growth cartilage was published by a Swiss anatomist Miescher in 1836. Subsequently, this structure was studied by a number of English, German and French anatomists and surgeons. This whole period was concluded by Alfred Kölliker´s extensive study of bone resorption and its significance for typical bone shapes and Karl Langer´s study of the vascular supply of the growing and mature bone. Research by French, English, German and Swiss scientists between 1727 and 1875 yielded fundamental insights into the growth of long bones, most of which are still valid today.

Property of Modified Bovine Bone Glue as an Environmental Additive in Water-Based Drilling Fluids.

At present, animal bone glue (BG) is being widely used in many fields, but there are no studies reported on oilfield chemistry. In this paper, an environmental water-based drilling fluids additive named bromoethane-modified bone glue (BG) was developed by using bovine bone glue and bromoethane as raw materials, anhydrous ethanol as solvent, sodium hydroxide as alkaline hydrolysis agent, and sodium carbonate as a system pH regulator. The inhibition, filtration performance, and temperature resistance of BG were evaluated. Performance study results show that the linear swelling rate of sodium bentonite (Na-MMT) was decreased from 50.2% (in tap water) to 38.2% (in 4 wt % BG solutions), and filtration loss was reduced from 30 mL (in tap water) to 12 mL (in 5 wt % BG). Hot-rolling experiments show that the BG solution still exhibits good performance even after 16 h × 130 °C. The reasons for BG to achieve excellent performance were analyzed through scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), ζ potential, thermogravimetric analysis (TGA), and microstructure. The results of SEM and FT-IR show that BG can fully dissolve in water and adsorb on the surface of clay particles by relying on its own adsorption functional groups such as -OH and -COOH. When 4% BG was added, ζ potential analysis revealed that the clay particle size declined by 0.502 μm, which indicated that BG can inhibit clay hydration swelling dispersion.

Tracing royal consumption and socio-symbolism through faunal remains: Zooarchaeology of Iron Age–Urartu Ayanis citadel, Eastern Turkiye

Despite having a research history of more than one and a half centuries, zooarchaeological investigations within Urartian archaeology are still very limited. This study marks a significant contribution presenting a comprehensive zooarchaeological dataset of 11,977 animal bones and fragments, primarily unearthed from a royal midden at the Urartian site of Ayanis citadel, Eastern Turkiye. The analysis encompasses species identification, age estimation, sex determination, and the identification of taphonomic and pathological changes. Rigorous measurements were taken to establish a robust zooarchaeological dataset. Logarithmic Size Index (LSI) was calculated to identify the morphological characteristics of Ayanis sheep, goats, and cattle, as well as potential sex-based exploitation of these animals. Contextualizing the results with faunal remains from contemporary Urartian sites including Bastam in Iran, and Karmir-Blur, Aramus and Horom in Armenia, this study presents hitherto overlooked archaeological evidence of animal-based consumption behavior and rituals, and possible animal taboos practiced by the Urartian royals and elites. It also offers new opportunities for comparative analyses and discussions for the biometric status of domestic livestock and animal-human interactions within the broader context of the Iron Age in the Lesser Caucasus and West Asia.

Domestic pig husbandry strategy during the Shuangdun Culture period (ca. 7300–6800 BP) from the stable isotopic perspective: A case study of the Yuhuicun site in Anhui Province, China

Shuangdun Culture, spanning from about 7300 to 6800 BP, was primarily distributed along the middle Huai River valley. This study aimed to elucidate the domestic pig husbandry strategy of Shuangdun Culture using stable carbon and nitrogen isotopes on animal bones from the Yuhuicun site. The results revealed that most of the Sus, displaying δ13C values from −20.5 to −17.1‰, had diets based on C3 plants, comparable to the diets of large and middle-sized deer whose δ13C values ranged from −20.7 to −18.1‰. Previous zooarchaeological investigations have suggested that domestic pigs were predominant among the faunal assemblage of the Yuhuicun site, with few wild boars and feral pigs present. However, the present study found no significant differences regarding the diet among the Sus population. Domestic pigs at Yuhuicun were inferred to be reared loosely and fed with wild plants, given that the human inhabitants primarily relied on gathering, with rice cultivation being small in scale. A comparison of pig husbandry strategies among the Yuhuicun, Houjiazhai, and Shuangdun sites revealed that most Sus from these three archaeological sites had similar diets. However, three Sus individuals with mixed diets of C3 and C4 plants were identified from the Shuangdun site, which may have been introduced from an outside area where millets were widely planted. The Shuangdun site is a central settlement in the middle Huai River valley, and its inhabitants likely had access to introduced food sources. This differentiation in the diet of domestic pigs is indicative of varying settlement patterns during this time period.

Effect of Bone Char Application on Soil Quality, Soil Enzyme and in Enhancing Crop Yield in Agriculture: A Review

Soil quality, in contrast to air or water, exhibits a heightened level of heterogeneity and necessitates closer examination due to its impact on the well-being of flora, fauna, and human beings. Organic carbon is considered a fundamental indicator of soil quality, as it plays a significant role in strategies aimed at mitigating climate change. The generation of bone char arises from a thermochemical conversion process involving defatted bones. Specific attention is focused on the solubility of P compounds, which serves to classify bone chars as potential slow-release P fertilizers. The introduction of P into the soil can be enhanced through an "internal activation" process facilitated by the adsorption of reduced S compounds. Additional properties of agronomic significance originate from the porosity of bone char, which promotes water retention and provides a habitat function for soil microorganisms. The evaluation of soil quality has been a longstanding practice, involving an examination of physical and chemical characteristics such as pH, nitrogen levels, soil organic carbon, bulk density, accessible water, aggregate stability, particle size distribution, and soil structure. Recently, the concept of soil quality has been expanded to encompass the notion of soil health, which is perceived as a finite, non-renewable resource that undergoes constant change. Research also demonstrates the crucial role of soil biota in the assessment of soil quality, as they exhibit rapid responsiveness to disturbances. Animal bones undergo a process of defatting, degelatinization, and subsequent incineration at temperatures ranging from 600-800°C to produce bone char (BC). Reports indicate that typical BC contains 152 g P kg<sup>-1</sup>, 280 g Ca kg<sup>-1</sup>, and 6.5 g Mg kg<sup>-1</sup>, with carbon content typically falling below 100 g kg<sup>-1</sup>. The solubility of bone char in the soil depends on factors such as pH and the soil's capacity to absorb P, situating it within the range between rock phosphate and triple super phosphate (TSP). The application of bone char to the soil can enhance soil health, resulting in increased crop yield and improved quality.

Mechanical and Architectural Changes in Animal Bone Following Fast Neutron Irradiation.

Damage to healthy bone following exposure to ionizing radiation has been well documented for at least seven decades. Among the reported effects are a transient increase in stiffness and a reduction in breaking strength. These changes have been linked to a decrease in osteoblast proliferation and differentiation, inducing cell cycle arrest, reducing collagen production, and increasing sensitivity to apoptotic agents. In this work, we analyzed some mechanical and structural changes in compact costal bovine bone (Hereford breed, n = 9) subjected to escalating doses of fast neutrons from a 7Li(p,n)7Be reaction. The mean neutron energy was 233 keV with calculated absorbed doses ranging from 0 to 4.05 ± 10% Gy. Samples were subjected to Young's Modulus (YM) and breaking strength testing with a Universal Testing Machine (UTM). We found an increase in Young's Modulus and a decrease in breaking strength as functions of increasing dose equivalent. Optical coherence tomography (OCT) revealed trabecular displacement into compact bone in an irradiated sample (D = 4.05 ± 10% Gy), with breaching of the endosteal wall. OCT further revealed a "crack-like" structure across the irradiated sample, potentially consistent with damage from a proton track resulting from an elastic (n,p) reaction. No previous report has been found on mechanical changes in large mammalian bones following fast neutron doses, nor of the OCT imaging of such samples.

Penelusuran sisa-sisa Kerajaan yang hilang dibalik Letusan Gunung Tambora di Situs Doro Bente

The archaeological research in the Tambora Peninsula was initiated by the National Archaeological Research Center in 2006, and from 2008 onwards, it was continued by the Archaeological Office in Denpasar-Bali until 2021. Numerous archaeological pieces of evidence have been successfully excavated from Mount Tambora's pyroclastic material. Recent research has been focused on the Doro Bente Site on the Teluk Saleh Peninsula. The research aims to understand the sequence of historical events on settlement centers based on artifact evidence resulting from the eruption of Mount Tambora in 1815. The study employs qualitative, descriptive methods, and a geoarchaeological approach. Data acquisition involves excavation, surveys, and literature studies. Stratigraphic analysis is conducted to identify sediment matrices and understand the deposition sequence. Excavation activities have uncovered significant artifacts such as ceramic fragments, pottery, animal bones, and shell fragments. Micro-scale deposition chronology in excavation box B28U18 indicates that the three lower layers, the oldest, were deposited in a marine environment, followed by five layers of pyroclastic material from the 1815 eruption of Mount Tambora. The geographical factors and available natural resources make Sumbawa Island a focal point for Chinese and European attention from the 13th to the 19th centuries AD.    

Development of New CD38 Targeted Peptides for Cancer Imaging.

Multiple myeloma (MM) affects over 35,000 patients each year in the US. There remains a need for versatile Positron Emission Tomography (PET) tracers for the detection, accurate staging, and monitoring of treatment response of MM that have optimal specificity and translational attributes. CD38 is uniformly overexpressed in MM and thus represents an ideal target to develop CD38-targeted small molecule PET radiopharmaceuticals to address these challenges. Using phage display peptide libraries and pioneering algorithms, we identified novel CD38 specific peptides. Imaging bioconjugates were synthesized using solid phase peptide chemistry, and systematically analyzed in vitro and in vivo in relevant MM systems. The CD38-targetedbioconjugates were radiolabeled with copper-64 (64Cu) with100% radiochemical purity and an average specific activity of 3.3 - 6.6MBq/nmol. The analog NODAGA-PEG4-SL022-GGS (SL022: Thr-His-Tyr-Pro-Ile-Val-Ile) had a Kd of 7.55 ± 0.291nM and was chosen as the lead candidate. 64Cu-NODAGA-PEG4-SL022-GGS demonstrated high binding affinity to CD38 expressing human myeloma MM.1S-CBR-GFP-WT cells, which was blocked by the non-radiolabeled version of the peptide analog and anti-CD38 clinical antibodies, daratumumab and isatuximab, by 58%, 73%, and 78%, respectively. The CD38 positive MM.1S-CBR-GFP-WT cells had > 68% enhanced cellular binding when compared to MM.1S-CBR-GFP-KO cells devoid of CD38. Furthermore, our new CD38-targeted radiopharmaceutical allowed visualization of tumors located in marrow rich bones, remaining there for up to 4h. Clearance from non-target organs occurred within 60min. Quantitative PET data from a murine disseminated tumor model showed significantly higher accumulation in the bones of tumor-bearing animals compared to tumor-naïve animals (SUVmax 2.06 ± 0.4 versus 1.24 ± 0.4, P = 0.02). Independently, tumor uptake of the target compound was significantly higher (P = 0.003) compared to the scrambled peptide, 64Cu-NODAGA-PEG4-SL041-GGS (SL041: Thr-Tyr-His-Ile-Pro-Ile-Val). The subcutaneous MM model demonstrated significantly higher accumulation in tumors compared to muscle at 1 and 4h after tracer administration (SUVmax 0.8 ± 0.2 and 0.14 ± 0.04, P = 0.04 at 1h; SUVmax 0.89 ± 0.01 and 0.09 ± 0.01, P = 0.0002 at 4h). The novel CD38-targeted, radiolabeled bioconjugates were specific and allowed visualization of MM, providing a starting point for the clinical translation of such tracers for the detection of MM.

The Early Upper paleolithic deposit of Mughr el-Hamamah (Jordan): Archaeobotanical taphonomy and site formation processes

With a rich, well-dated Early Upper Palaeolithic layer, the Mughr el-Hamamah cave site is key for understanding the Middle-Upper Palaeolithic transition in the Levant. The archaeological deposit consists of two units. Layer A resulted from pastoral activities during the 20th century and Layer B dated between 44.5 and 40.0 ky BP. During Layer A’s formation, shepherds disturbed Layer B, redepositing Early Upper Palaeolithic sediments and lithic artefacts in Layer A matrix. Activity from Layer A’s formation also resulted in spatially patchy percolation and bioturbation, leaving microarchaeological traces such as dung spherulites in some areas in Layer B. In contrast, contemporaneous chemical diagenetic processes from Layer B’s primary formation caused spatially uneven post-depositional dissolution of animal bone. In this article we present a multi-proxy microarchaeological approach to investigate the post-depositional processes in Layer B, focussing on possible impacts on the plant archaeological record. The identification of intrusive spherulites from shepherds’ activities define the limits of disturbance in Layer B. Micromorphological analyses have identified four intact micro-facies in Layer B, representing an interplay of natural and anthropogenic factors. Micromorphological details in bedded combustion features favour the interpretation that associated phytoliths represent fuel traces. Dicot fruit phytoliths occur in the western area of the cave, where well-preserved charred wood and seeds were also found. Grass-diagnostic phytoliths correspond to C3 and C4 taxa, indicating an overall humid environment with dry spells. Microarchaeological analysis identifies traces of both bedded and dispersed hearth materials, mixed with variable plant resources for food, fuel, and possibly other uses. This strengthens the interpretation of Mughr el-Hamamah Layer B as a dense, complicated palimpsest of recurring activities, formed over many millennia.

Plant Remains and What Else? Environmental Archaeology of the Late Hellenistic Pit Fills of Sexaginta Prista, Northern Bulgaria

ABSTRACT This study combines several categories of environmental remains retrieved from machine-assisted water flotation samples, originating from the pit field of the Late Hellenistic archaeological site of Sexaginta Prista (2c. BC – 1c. AD), located on the River Danube in North Bulgaria. The analysis of the different organic remains, such as archaeobotanical macro-remains (seeds, wood charcoal and ‘food crusts’), fish scales, molluscs and animal bones, aims at exploring the possible utilisation scenarios of the pits, including crop/food storage and preparation, disposal activities and ritual practices and also allowed for a preliminary past-environmental reconstruction of the site during the study of interest.

Astatine-211 Radiopharmaceuticals; Status, Trends, and the Future

Abstract: The low range of alpha particles provides an opportunity to better target cancer cells theoretically leading to the introduction of interesting alpha emitter radiopharmaceuticals including 225Ac, 212Pb, etc. The combination of high energy and short range of alpha emitters differentiates targeted radiotherapy from other methods and reduces unwanted cytotoxicity of the cells around the tumoral tissue. Among interesting alpha emitters candidates for targeted therapy, 211At, one of the radioisotopes with the best optimal decay properties, shows great promise for targeted radiotherapy in some animal prostate cancer xenograft studies and bone micro tumors with significant effects compared to other beta and alpha emitters and also demonstrates interesting properties for clinical applications. However, production and application of this alpha emitter in the development of actinium-based radiopharmaceuticals is hampered by many obstacles. This mini-review demonstrates 211At production methods, chemical separation, radiolabeling procedures, 211At-radiopharmaceuticals and their clinical trials, transport, logistics, and costs and future trends in the field for ultimate clinical applications. This review showed that there are limited clinical trials on 211Ac-based radiopharmaceuticals, which is due to the low accessibility of this radioisotope and other limitations. However, the development programs of major industries indicate the development of 211Ac-based radiopharmaceuticals in the future.

Nomogram for Predicting the Risk of Complications after Endoscopic Foreign Body Removal from the Adult Upper Gastrointestinal Tract.

Ingested foreign bodies may become impacted in the upper gastrointestinal tract, requiring endoscopic removal. To establish and validate a nomogram to determine the risk of complications following endoscopic foreign body removal. We retrospectively analyzed the data of 1510 adult patients who underwent endoscopic removal of ingested foreign bodies between January 2019 and December 2022. All participants were randomly allocated in a 7:3 ratio to the training (n = 1057) and validation (n = 453) cohorts. A nomogram for the development of major complications associated with endoscopic foreign body removal was established based on risk factors identified by logistic regression analysis. Four independent risk factors for the development of major complications were identified by multivariate regression analysis: older age, impaction time > 24h, type of foreign body (animal bones and jujube pits), and number of pressure points exerted on the digestive tract wall (one and ≥ two). The nomogram constructed using these factors showed favorable discriminatory values, with an area under the curve of 0.76 (95% confidence interval, 0.73-0.78) in the training cohort and 0.74 (95% confidence interval, 0.72-0.76) in the validation cohort. Older patients who ingested bones or jujube pits with more pressure points exerted on the digestive tract wall more than 24h earlier should be considered most at risk of major complications after endoscopic removal of foreign bodies. The nomogram established in this study can be conveniently used to assess patients and develop treatment plans for the management of foreign body ingestion.

Prevalence of drifting osteons distinguishes human bone.

The histological, or microscopic, appearance of bone tissue has long been studied to identify species-specific traits. There are several known histological characteristics to discriminate animal bone from human, but currently no histological characteristic that has been consistently identified in human bone exclusive to other mammals. The drifting osteon is a rare morphotype found in human long bones and observationally is typically absent from common mammalian domesticates. We surveyed previously prepared undecalcified histological sections from 25 species (human n = 221; nonhuman primate n = 24; nonprimate n = 169) to see if 1) drifting osteons were indeed more common in humans and 2) this could be a discriminating factor to identify human bone histologically. We conclude that drifting osteons are indeed more prevalent in human and nonhuman primate bone relative to nonprimate mammalian bone. Two criteria identify a rib or long bone fragment as human, assuming the fragment is unlikely to be from a nonhuman primate given the archaeological context: 1) at least two drifting osteons are present in the cross-section and 2) a drifting osteon prevalence (or as a percentage of total secondary osteons) of ≥ 1%. We present a quantitative histological method that can positively discriminate human bone from nonprimate mammalian bone in archaeological contexts.

Physical Characteristics of Fishbone Gelatin (Gel Strength, Viscosity, and pH): Review

Gelatin is a polypeptide molecule derived from collagen, the main protein that forms the skin and bones in animals. Gelatin serves various functions in the food industry, acting as a stabilizer, whipping agent, gel-former, and binder agent. Research on utilizing fish bones for gelatin production has been widely conducted. Gel strength, viscosity, and pH are some of the physical characteristic parameters of fish bone gelatin. Gelatin's ability to transition between the gel and sol phases, or its reversible nature, is reflected in its gel strength. Viscosity of gelatin reflects the molecule's ability to flow in solutions, whether they are simple organic liquids or dilute suspensions, playing a crucial role in maintaining emulsion stability. The acidity level (pH) in fish bone gelatin is influenced by the pH of the hydrolysis or extraction solution, while the source of gelatin, acidity level, extraction method, temperature, and duration also impact the gel strength and viscosity values. Therefore, there is a need for a study on the physical characteristics such as gel strength, viscosity, and pH of fish bone gelatin.

Thermochemical Conversion of Animal-Derived Waste: A Mini-Review with a Focus on Chicken Bone Waste

Food waste, particularly animal-derived waste, presents a significant challenge globally, prompting the need for sustainable management strategies. In 2022, the amount of food waste per capita reached 131 kg/capita in the EU (European Union), which is why the search for environmentally friendly ways to manage food waste through thermochemical conversion processes has gained momentum in recent years. Animal-derived waste is a good source of organic matter (proteins, lipids, and polysaccharides) and mineral compounds (calcium phosphate, mostly hydroxyapatite). This composition makes animal-derived waste valuable for the extraction of chemical compounds, such as hydroxyapatite (HAp), which constitutes up to 70 wt% of animal bones; keratin; collagen; and hyaluronic acid (HA), to produce pharmaceutical, medical, or industrial by-products. The thermochemical conversion of chicken bones through pyrolysis and gasification creates a new opportunity to valorize this type of waste by reintroducing valuable by-products into the economy and thus achieving sustainable waste management objectives. The results of this study showcase the multiple applications of the pyrolysis of chicken bone waste products (as adsorbents in aqueous mediums, catalysts, fertilizers, and biomedical applications) and the necessity of a better exploration of the gasification process of chicken bone waste. Therefore, this study explores the properties of animal-derived waste and discusses the pyrolysis and gasification of chicken bone waste, the influence of process conditions on product yields, and the catalytic enhancement of these thermochemical processes.

Efficacy Study of Eco-Friendly Bleaching Agent for Skeleton Preservation in Animal Skeleton

The currently used bleaching materials in skeletal preservation are hydrogen and carbamide peroxide materials with specific concentrations and techniques that impact environmental sustainability. This research describes a simple and eco-friendly technique for preserving skeletons. This research used a qualitative approach. Data were collected through observation and documentation. The collected data were analyzed descriptively. The results show that natural extracts, such as lemon combined with baking soda and commercial bleaching cloth agent, can bleach the skull and bone to preserve the skeleton. The commercial bleaching cloth agent is more capable of bleaching animal skulls and bones to preserve skeletons than natural extracts combined with baking soda do; however, the result is more brittle skeletons. Although specimens in lemon extract combined with baking soda solution more slowly clean and bleach skeleton than commercial bleaching solution, skeletal animals have bond strength and environment-friendly processes. This study recommends that an eco-friendly bleaching agent for skeletal preservation should be applied because it can cope with biological practices about osteology and other science subjects.